Ku70 and Ku80 have also been experimentally characterized in plants, where they appear to play a similar role to that in other eukaryotes. In rice, suppression of either protein has been shown to promote homologous recombination (HR) [13] This effect was exploited to improve gene targeting (GT) efficiency in Arabidopsis thaliana .

Fig. 1. Spermatogenesis in Ku70 and Ku70 Rap1 knockout mice. (A) The testes of Ku70 2 /and Ku70 2/ Rap1 D mice are of reduced size, as shown for wild-type (left) and Ku70 2/ (right) testes. Scale bar: 5 mm. (B) Ku70 2/ and Ku70 2/ Rap1 D/ (dko) mice display a significant (P,0.001) reduction in testis size compared with wild type (WT) and

Securin 2021-02-22 · Acetylation modulates DNA-mediated Ku70-dependent IFN-λ1 induction. Ku80 colocalizes with Ku70 during Ku70 translocation from the nucleus to the cytoplasm, but not directly involving in Ku70's cytosolic DNA activity. The third research area is to study the host genetic determinants that affect HIV pathogenesis and progression. 2017-03-01 · Ku70 Antibody (G-4) is a mouse monoclonal IgG 2b (kappa light chain) provided at 200 µg/ml specific for an epitope mapping between amino acids 564-595 at the C-terminus of Ku70 of mouse origin recommended for detection of Ku70 of mouse, rat and human origin by WB, IP, IF and ELISA The two characteristic KU70/KU80 functional domains were detected in both BC1G_16015.1 and BC1G_10199.1 proteins: the KU70/KU80 N-terminal α/β domain (Interpro entry IPR005161), which is similar to the von Willebrand factor type A domain (Interpro entry IPR002035), and the central KU70/KU80 β-barrel domain (Interpro entry IPR006164). Plasmid pEGFP-C1-FLAG-Ku70 from Dr. Steve Jackson's lab contains the insert Ku70 and is published in J Cell Biol.

Ku70

This antibody reacts with human, rat samples. Cat.No. 10723-1-AP. KD/KO Validated. KU70. Education . Community See All. 1,579 people like this.

Ku70 Antibody (PA5-27538) in WB Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK293 (Lane 4) and HepG2 (Lane 5).

Ku70 Antibody (MA5-32645) in WB Western blot was performed using Anti-Ku70 Recombinant Rabbit Monoclonal Antibody (JM61-31) (Product # MA5-32645) and a 70kDa band corresponding to Ku70 was observed across cell lines tested. Ku70 Antibody (PA5-27538) in WB Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK293 (Lane 4) and HepG2 (Lane 5). Ku70 is a novel Mcl-1 deubiquitinase that could be a potential target for cancer therapy by manipulating Mcl-1 deubiquitination. loss of expression correlates with decreased survival in gall bladder malignancies patients SMAR1-mediated regulation of repair and apoptosis via a complex crosstalk involving Ku70, HDAC6 and Bax. Single-stranded DNA-dependent ATP-dependent helicase.

Plasmid pEGFP-C1-FLAG-Ku70 from Dr. Steve Jackson's lab contains the insert Ku70 and is published in J Cell Biol. 2013 Jul 29. This plasmid is available through Addgene.

Denna blankett ska användas av den som ska lämna kontrolluppgift avseende näringsbidrag och royalty. Ku70 and Ku80 have also been experimentally characterized in plants, where they appear to play a similar role to that in other eukaryotes. In rice, suppression of either protein has been shown to promote homologous recombination (HR) [13] This effect was exploited to improve gene targeting (GT) efficiency in Arabidopsis thaliana . Ku70 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Ku70 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Results 2.1. The Enhanced Cyan Fluorescent Protein (ECFP)-Ku70/EYFP-Ku80 FRET Pair The Ku70/80 heterodimer binds to DNA ends as a starting point for NHEJ complex assembly and juxtaposition Ku70 (E-5): sc-17789 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku70 (p70) and Ku86 (p86) which is also known by the synonym Ku80 or (p80). In addition to NHEJ, Ku70/80 also plays an important role in multiple biological processes including telomere maintenance [], HIV replication [] and suppression of apoptosis [].
Henrik laurell

Ku70 Antibody (PA5-27538) in WB Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK293 (Lane 4) and HepG2 (Lane 5). Ku70 is a novel Mcl-1 deubiquitinase that could be a potential target for cancer therapy by manipulating Mcl-1 deubiquitination. loss of expression correlates with decreased survival in gall bladder malignancies patients SMAR1-mediated regulation of repair and apoptosis via a complex crosstalk involving Ku70, HDAC6 and Bax. Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair.

The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro.
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KU70. Education . Community See All. 1,579 people like this. 1,603 people follow this. About See All. Education. Page Transparency See More. Facebook is showing

Kontrolluppgiftsblanketter. Det finns ett flertal olika kontrolluppgiftsblanketter i inkomstslaget tjänst: KU10 (lön och förmåner m.m.), KU13 (SINK), KU14 (pensionsgrundande inkomst m.m.), KU16 (sjöinkomst), KU17 (SINK med sjöinkomst), KU18 (pensions- och försäkringsutbetalare) och KU19 (pensions- och försäkringsutbetalare med SINK).

Dessa uppgifter finns inte med i e-tjänsten Lämna kontrolluppgifter. Ku70 and Ku80 make up the Ku70/80 heterodimer. They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes. The stability of these proteins depends on each other.

Ku70 and Ku80 form a pseudosymmetrical heterodimer with a preformed ring (also called DNA aperture) responsible for sequence‐independent DNA binding. Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Dec 5, 2006 Accumulation of XRCC4/ligase IV on DSBs depended on the presence of Ku70/ 80, but not DNA-PKCS. We detected a direct interaction between Feb 27, 2014 The KU70-deficient mutant generated herein was effective in improving gene deletion frequency and allowed shorter homology sequences to Monoclonal Antibody for studying Ku70 in the Cell Cycle / Checkpoint research area.